Root rot of tea tree is one of the major diseases affecting tea production and poses a serious threat to tea yield and quality. In order to investigate the screening of biocontrol microorganisms in tea plantation soil and their inhibitory effects on tea root rot, this study was conducted to isolate and characterize biocontrol microorganisms from tea plantation soil, and to screen Z-1 strain and evaluate its inhibitory effects on tea root rot. The study examined the inhibitory ability of Z-1 strain against the pathogenic fungi of tea tree root rot using plate standoff assay and found that its inhibition rate was as high as 42.9%. Further field trials showed that the Z-1 strain had a positive effect on tea tree growth, with root length, plant height and fresh weight showing significant improvement. Compared with traditional chemical control methods, Z-1 strain showed comparable control effects and lower toxicity. The study suggests that Z-1 strain has strong potential for disease resistance and can provide new ideas and methods for green control of root rot of tea tree.