With the in-depth implementation of rural revitalization strategy, rural landscape design not only requires ecological aesthetic function, but also needs to focus on improving the efficiency and comfort of human activities. Biomechanics, as a discipline that studies human movement and mechanics, provides important theoretical support for landscape design in rural revitalization. This paper combines the principles of biomechanics and explores its application in landscape design for rural revitalization, especially in the areas of trail design, agricultural landscape optimization and its impact on farmers’ operational efficiency and labor consumption. Through field experiments and data analysis, this paper analyzes the effects of different slopes, path widths, and material choices on walking speeds, stride lengths, muscle burdens, operating time, and labor intensity. The results show that appropriate slope (within 5%) and wide road (above 2.5 m) can significantly improve walking efficiency and reduce physical exertion, while the optimized agricultural road design effectively improves operational efficiency and reduces farmers’ fatigue. In addition, through the optimized design of walking paths and agricultural roads, combined with biomechanical models, the harmonious symbiosis of people, environment and architecture can be achieved, and the overall effect of the rural revitalization project can be enhanced. The research in this paper provides empirical support for the application of biomechanics in rural landscape design, and proposes new design directions and practical solutions for the sustainable development of rural landscapes in the future.