Chinese-American adolescents’ cultural identity problems have become increasingly prominent in the context of globalization, and traditional intervention methods have limited effects. In this study, we used eye-tracking technology combined with electroencephalography EEG measurements to construct an emotional arousal experiment in the ancestral house VR scene, and recruited 20 Chinese adolescent subjects for a cultural identity intervention study. Through the ETG 2wTM eye-tracker of SMI (Germany) and the GES 400 EEG system of EGI (USA), the physiological data of the subjects in different emotional scenes were collected, and the subjective emotional responses were assessed by using the SAM scale. The results showed that the subjects’ pupil diameter during positive VR scene interaction was 4.362, which was significantly larger than that of neutral emotion, 4.049; the affective valence of positive emotional behaviors reached 7.163, and arousal was 7.162, both of which were significantly higher than that of the neutral condition; the Chinese adolescent ethnic identity was 5.52, and 66% of the subjects wished to maintain their perceived Chinese ethnicity; and the dimensions of ethnic achievement identity and learning motivation were significantly correlated at the 0.01 level. The study shows that the ancestral house VR scene can effectively stimulate the emotional resonance of Chinese adolescents, provide a technological path for cultural identity intervention, and is of great significance in promoting the overseas transmission of Chinese culture.