At present, Jiujiang Xunongyuan Beekeepers Professional Cooperative presents the characteristics of stable improvement of production scale and production capacity, and continuous improvement of industrial chain. This paper takes Jiujiang Xunongyuan Beekeepers Professional Cooperative as a research sample to explore the realization path of rural revitalization under the trend of integration of agriculture, culture and tourism. On the assessment of the development of the agricultural, cultural and tourism industry, a set of agricultural, cultural and tourism industry indicator system containing a total of 16 indicator systems is systematically, dynamically and scientifically constructed. Based on the development data of the agricultural, cultural and tourism industry in Jiujiang City from 2011 to 2020, the correlation data of agriculture and tourism are calculated, and the entropy weight method is used to determine the weights of the indicators. Using the comprehensive evaluation model and the coupling coordination degree model method, the interactive coupling mechanism of agriculture, culture and tourism in Jiujiang City is built. Combined with its dynamic evolution characteristics, the analysis concludes that a total of four indicators, namely, gross agricultural product (billion yuan), domestic tourism revenue (billion yuan), the number of employees in the cultural industry (10,000 people), and the operating profit of the cultural industry (billion yuan), are the important influencing factors of the coupling and coordination degree of the integration of agriculture, culture, and tourism and the revitalization of the countryside. In the three time nodes of 2011, 2015 and 2020, the gray correlation between the two systems is greater than 0.500.